Heparinases

Using high purity enzymes will improve the quality of your results.

IBEX produces a family of high purity recombinant glycosaminoglycan (GAG) lyases; among them are Heparinase I, Heparinase II, Heparinase III, which are widely used in research, the production of low molecular weight heparin, and in conjunction with clinical for haemostasis monitoring devices

IBEX has developed a proprietary Flavobacterium heparinum  expression system which unlike other heparinase expression systems produces glycosylated enzymes.

Competitor enzymes purified from the native strain of Flavobacterium heparinum (also known as Pedobacter heparinus) will almost certainly contain cross-contaminants of other GAG enzymes.

Due to its high purity and reaction time IBEX Heparinase I is the only heparinase used in FDA and EU approved hemostasis-measuring Point Of Care devices.

OUR PRODUCTS

HEPARINASE I

Click the image above to learn more. Heparinase I cleaves heparin, and to a lesser extent, heparan sulfate. Available as Liquid (Frozen) or Lyophilized. Click the data sheets .pdf icons below for complete details.

Heparinase II

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Heparinase II (EC unassigned) cleaves both heparin and heparan sulfate. Available as Liquid (Frozen) or Lyophilized. Click data sheets below for complete details.

HEPARINASE III

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Heparinase III (EC 4.2.2.8) cleaves heparan sulfate exclusively. It does not cleave unfractionated or low molecular weight heparins. Click below for complete details.

OVERVIEW

Heparin and heparan sulfate (HS) glycosaminoglycans are linear sulfated polysaccharides located on cell-surface membranes and in extracellular matrices in virtually all animal tissues. Heparin and HS have been implicated in cell-biological processes, cell adhesion and regulation of enzymatic catalysis (1). HS chains have been shown to interact with a variety of growth factors, chemokines, ECM proteins, and enzymes, including antithrombin, fibroblast growth factors and vascular endothelial growth factor (2). Heparin has been widely used as an anticoagulant drug (3,4), and it has been shown to regulate cellular process by binding, stabilizing and activating various growth factors (5).

References

  1. Fritz, T. et al. (1994)  Biol. Chem.269, 28809-28814. PMID: 7759502
  2. Linhardt, R.J. et al. (1990) Biochemistry29, 2611-2617. PMID: 2334685
  3. Linhardt, R.J., and Gunay, N.S. (1999)  Thromb. Hemost. 25 Suppl 3, 5-16. PMID: 10549711
  4. Casu, B. et al. (2002) Biochemistry41, 10519-10528. PMID: 15106730
  5. Knudsen, C.B. and Knudsen, W. (2001) Cell Dev. Biol.12, 69-78. PMID: 11292372